APPLICATION CLOSING DATE: 30.10.2025 Human Technopole (HT) is an interdisciplinary life science research institute, created and supported by the Italian Government, with the aim of developing innovative strategies to improve human health. Its mission is to conduct cutting-edge research and translate innovations into methodologies accessible to the entire national scientific community. As part of this mission, HT provides access to state-of-the-art research infrastructure in five key areas through the National Facilities for Structural Biology, Light Imaging, Genomics, Genome Engineering, and Data Handling & Analysis. About the Dynamic Single Molecule (DSM) Unit: The Dynamic Single Molecule (DSM) Unit operates within the National Facility for Structural Biology; together with other units serves as a scientific and technological hub for integrative structural biology. The unit offers advanced tools to visualize biological processes in real time at the single-molecule level. It is built around two cutting-edge Lumicks instruments, integrating optical tweezers with fluorescence-based and label-free detection modules. Fluorescence imaging enables high spatial and temporal resolution observation of labeled molecules, while optical tweezers uniquely allow for controlled perturbation of biological systems by applying precise picoNewton (pN) forces. This integrated approach complements high-resolution structural studies, unveiling the dynamic mechanisms underlying molecular processes. The goal of the Unit is to establish standardized workflows, from sample preparation to data analysis. The Unit not only provides instrument access and training but also supports scientists in developing and refining their experimental ideas. We are seeking a highly skilled and motivated Single Molecule Specialist to join the National Facility for Structural Biology. The ideal candidate will possess deep expertise in single-molecule techniques and play a pivotal role in supporting research projects, developing novel methodologies, and collaborating with interdisciplinary teams to drive HT’s vision of scientific excellence forward. The candidate will be reporting to the Manager of the Unit. This role offers an exciting opportunity to contribute to pioneering research in single-molecule studies while collaborating with top-tier scientists and utilizing cutting-edge technology. As part of the onboarding process, we will offer comprehensive training and a clear path for professional growth, making this a great opportunity to develop your expertise in the field. If you are passionate about advancing structural biology through single-molecule techniques, we encourage you to apply. Key tasks and responsibilities User Support & Training: Provides hands-on technical support and user training, guides experimental set-up and data acquisition Experimental Design & Operations: Designs, implements, and optimizes single-molecule assays, conducts advanced experiments, troubleshoots technical challenges, and implements new applications to expand the facility’s capabilities. Collaboration & Scientific Consultancy: Serves as a key liaison with internal teams and external users, advises on experimental design and project execution, and contributes to publications, data analysis, and grant applications. Equipment & Resource Management: Ensures optimal operation of laboratory instruments, coordinates procurement and supplies to maintain efficiency and operational continuity. Job requirements Essential requirements McS in Biophysics, Chemistry, Molecular Biology, Biochemistry or Bioengineering, or a related field Proven hands-on experience (including postgraduate, postdoctoral or industry) Fluency in English Expertise in at least one of the listed techniques: single-molecule fluorescence microscopy, single molecule FRET, single molecule tracking, optical tweezers, atomic force microscopy (AFM) Preferential requirements Experience in operating a Lumicks instrument Basic programming skills (Python) Knowledge in biochemical/biophysical characterisation of macromolecular samples Experience in protein expression and purification, and labelling Background in structural biology Ph.D. in Biophysics, Chemistry, Molecular Biology, Biochemistry or Bioengineering, or a related field Soft Skills: Strong problem-solving and critical-thinking skills Excellent communication and collaboration abilities Capacity to work in a multidisciplinary environment and lead research initiatives Application Instructions Please apply online by submitting a single document that includes: a CV. a motivation letter in English relating your track record to the specifics of the call. names and contacts of 2 referees. Why Human Technopole HT seeks scientific excellence, we recruit the best scientific talents through international, open calls. Our working environment is international, friendly and inclusive. Our scientists work together across disciplines on research topics of biomedical relevance, leveraging synergies between their diverse skillsets and methodological approaches. We believe that highly diverse teams yield the best and most innovative results. We engage in outward-facing scientific activities aimed at benefiting the national and international research community. Training is also at the heart of our activity, with initiatives and opportunities for our staff, including scientific courses, conferences and workshops. Main benefits Welfare plans. Meal delivery service. Work-life balance provisions. Italian language training for foreigners. Parental leave up to 1 year and other support for new parents. Flexible working hours. Remote working policy. Support for relocation. Researchers coming to Italy for the first time, or returning after residing abroad, benefit from very attractive income tax benefits. Special consideration will be given to candidates who are part of the protected categories list, according to L. 68/99. Number of positions offered: 1 Contract offered: CCNL Chimico Farmaceutico, Fixed-term 3 years with possibility of renewal -Employee Level Salary range: up to 41K, depending on the seniority of the candidate. Tax benefits where applicable. The position is based in Milan. “The Foundation reserves the right, at its sole discretion, to extend, suspend, modify, revoke, or cancel this job posting without giving rise to any rights or claims whatsoever in favor of the candidates; the Foundation reserves, however, the right not to proceed with the awarding of the above-described assignment due to the effect of supervening regulatory provisions and/or obstructive circumstances”.