Rottapharm Biotech, a research company dedicated to the discovery and development of innovative drugs in various therapeutic areas such as rheumatology, pain and inflammation, immunology, and immuno-oncology, is seeking a researcher to join the R&D team. The researcher will be involved in the discovery and development of next-generation AAV capsids for use in gene therapies targeting osteoarthritis (OA) tissues.
Key Responsibilities :
* Prepare and maintain cell cultures (including primary cell cultures), perform studies and experiments such as tissue processing, biochemical analyses, DNA, RNA, and protein isolation, qRT-PCR, ELISA, and immunoblot processing.
* Plan and execute in vitro and in vivo screening campaigns in human OA tissues and animal OA models; analyze biodistribution and transduction profiles across tissues.
* Develop and implement in vitro screening assays, including binding assays and transduction readouts in relevant cell lines.
* Collaborate with cross-functional teams on vector design, production, and payload optimization.
* Analyze data and manage experimental results.
* Maintain accurate records of experimental design and outcomes; contribute to internal reports, presentations, and publications.
Qualifications :
* BSc, MSc, or PhD in a relevant subject such as Virology, Molecular Biology, or Cell Biology with an AAV focus.
* Strong molecular biology and cell culture skills, including mammalian cell culture (stable and adherent lines), vector engineering, genetic engineering, PCR, transfection, transduction, and assay development.
* Strong foundation in AAV biology and capsid structure-function.
* Experience with high-throughput screening and NGS-based analysis is preferred.
* Prior work with in vivo models is preferred.
* Experience in FACS analysis and IHC is a plus.
* Excellent analysis, accuracy, confidentiality skills, and a strong attitude to achieve company goals.
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